Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

JoVE 3308 1/13/2012

Michael T. Kuhlmann¹, Simon Cuhlmann^2,3, Irmgard Hoppe¹, Rob Krams³, Paul C. Evans², Gustav J. Strijkers⁴, Klaas Nicolay⁴, Sven Hermann¹, Michael Schäfers¹

¹European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, ²British Heart Foundation Cardiovascular Sciences Unit, Imperial College London , ³Department of Bioengineering, Imperial College London , ⁴Biomedical Engineering, Eindhoven University of Technology

The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.

Introducing Shear Stress in the Study of Bacterial Adhesion

JoVE 3241 9/02/2011

Magali Soyer, Guillaume Duménil

Blood vessels as a target for infection, Paris center for cardiovascular research, INSERM U970

During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

JoVE 2640 2/13/2011

Chia-Hua Lee¹, Suman Bose², Krystyn J. Van Vliet¹, Jeffrey M. Karp³, Rohit Karnik²

¹Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, ²Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, ³HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School

We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

JoVE 3349 1/17/2012

Whitney O. Lane¹, Alexandra E. Jantzen², Tim A. Carlon², Ryan M. Jamiolkowski³, Justin E. Grenet¹, Melissa M. Ley¹, Justin M. Haseltine², Lauren J. Galinat², Fu-Hsiung Lin¹, Jason D. Allen⁴, George A. Truskey², Hardean E. Achneck¹

¹Department of Surgery, Duke University Medical Center, ²Department of Biomedical Engineering, Duke University , ³School of Medicine, University of Pennsylvania , ⁴Department of Medicine, Division of Cardiology, Duke University Medical Center

We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

JoVE 1009 2/11/2009

Georg Wiese¹, Steven R. Barthel², Charles J. Dimitroff²

¹Department of Dermatology, Brigham and Women's Hospital, ²Department of Dermatology, Brigham and Women's Hospital and Harvard Medical School

This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.

January 2012: This Month in JoVE

JoVE 4194 1/02/2012

Aaron Kolski-Andreaco

September 2011: This Month in JoVE

JoVE 3877 9/01/2011

Aaron Kolski-Andreaco

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

JoVE 2690 4/29/2011

Rebecca R. Vanderpool, Naomi C. Chesler

Department of Biomedical Engineering, University of Wisconsin – Madison

The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

JoVE 2691 4/08/2011

Kee-Won Lee¹, Yadong Wang^1,2

¹Department of Bioengineering, University of Pittsburgh, ²McGowan Institute for Regenerative Medicine, University of Pittsburgh

Elastomeric PGS scaffolds with vascular smooth muscle cells cultured in a pulsatile flow bioreactor may lead to promising small-diameter arterial constructs with native ECM production in a relatively short culture period.

Isolation of Valvular Endothelial Cells

JoVE 2158 12/29/2010

Russell A. Gould, Jonathan T. Butcher

Department of Biomedical Engineering, Cornell University

We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.