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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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 JoVE Applied Physics

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

1Department of Physics, University of Ottawa, 2Ottawa-Carleton Institute of Biomedical Engineering, University of Ottawa


JoVE 51081

A methodology for preparing solid-state nanopores in solution for biomolecular translocation experiments is presented. By applying short pulses of high electric fields, the nanopore diameter can be controllably enlarged with subnanometer precision and its electrical noise characteristics significantly improved. This procedure is performed in situ using standard laboratory equipment under experimental conditions.

 JoVE Bioengineering

Bridging the Bio-Electronic Interface with Biofabrication

1Fischell Department of Bioengineering, University of Maryland, 2Institute for Bioscience and Biotechnology Research, University of Maryland, 3Department of Materials Science and Engineering, University of Maryland


JoVE 4231

This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.

 JoVE Applied Physics

Development of a 3D Graphene Electrode Dielectrophoretic Device

1Department of Chemical Engineering, Michigan Technological University, 2Department of Mechanical Engineering, Michigan Technological University, 3XG Sciences, Inc.


JoVE 51696

A microdevice with high throughput potential is used to demonstrate three-dimensional (3D) dielectrophoresis (DEP) with novel materials. Graphene nanoplatelet paper and double sided tape were alternately stacked; a 700 μm micro-well was drilled transverse to the layers. DEP behavior of polystyrene beads was demonstrated in the micro-well.

 JoVE Applied Physics

Optical Trapping of Nanoparticles

1Electrical and Computer Engineering, University of Victoria


JoVE 4424

The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.

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 JoVE Neuroscience

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

1Institute for Microstructural Sciences, National Research Council of Canada, 2Institute for Biological Sciences, National Research Council of Canada, 3Hotchkiss Brain Institute, University of Calgary


JoVE 3288

We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

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 JoVE Bioengineering

Monitoring Protein Adsorption with Solid-state Nanopores

1Department of Physics, Syracuse University


JoVE 3560

A method of using solid-state nanopores to monitor the non-specific adsorption of proteins onto an inorganic surface is described. The method employs the resistive-pulse principle, allowing for the adsorption to be probed in real-time and at the single-molecule level. Because the process of single protein adsorption is far from equilibrium, we propose the employment of parallel arrays of synthetic nanopores, enabling for the quantitative determination of the apparent first-order reaction rate constant of protein adsorption as well as and the Langmuir adsorption constant.

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 JoVE Clinical and Translational Medicine

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

1Department NeuroFarBa, Division of Pharmacology, University of Florence, 2Department of Clinical and Experimental Medicine, Division of Physiology, University of Florence


JoVE 51116

Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.

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 JoVE Biology

Introduction to Solid Supported Membrane Based Electrophysiology

1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt


JoVE 50230

Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.

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 JoVE Applied Physics

Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities

1School of Physics & Astronomy, University of St Andrews


JoVE 50216

Use of photonic crystal slow light waveguides and cavities has been widely adopted by the photonics community in many differing applications. Therefore fabrication and characterization of these devices are of great interest. This paper outlines our fabrication technique and two optical characterization methods, namely: interferometric (waveguides) and resonant scattering (cavities).

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 JoVE Neuroscience

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

1Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin


JoVE 51706

Cortical networks are controlled by a small, but diverse set of inhibitory interneurons. Functional investigation of interneurons therefore requires targeted recording and rigorous identification. Described here is a combined approach involving whole-cell recordings from single or synaptically-coupled pairs of neurons with intracellular labeling, post-hoc morphological and immunocytochemical analysis.

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