Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

JoVE 4112 5/15/2012

Conrad T. Pfeiffer, Christian E. Schafmeister

College of Science and Technology, Temple University

The efficient solid-phase peptide synthesis of a functionalized bis-peptide trimer utilizing a "safety catch" cleavage procedure from HMBA resin is described.

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

JoVE 3373 11/02/2011

Helen Tran, Sarah L. Gael, Michael D. Connolly, Ronald N. Zuckermann

Molecular Foundry, Lawrence Berkeley National Laboratory

A simple and general manual peptoid synthesis method involving basic equipment and commercially available reagents is outlined, enabling peptoids to be easily synthesized in most laboratories. The synthesis, purification and characterization of an amphiphilic peptoid 36mer is described, as well as its self-assembly into highly-ordered nanosheets.

November 2011: This Month in JoVE

JoVE 4025 11/01/2011

Aaron Kolski-Andreaco

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

JoVE 3030 9/29/2011

Min Wei, Federica Madia, Valter D. Longo

Andrus Gerontology Center, Department of Biological Sciences, Department of Molecular and Computational Biology, University of Southern California, Los Angeles

Here we describe a set of DNA mutation assays that can be combined with the yeast chronological life span model to study the genes/pathways that regulate or contribute to genomic DNA instability during aging.

Metabolic Pathway Confirmation and Discovery Through ¹³C-labeling of Proteinogenic Amino Acids

JoVE 3583 1/26/2012

Le You¹, Lawrence Page², Xueyang Feng¹, Bert Berla¹, Himadri B. Pakrasi³, Yinjie J. Tang¹

¹Department of Energy, Environmental and Chemical Engineering, Washington University, ²Department of Biology, Washington University, ³Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

¹³C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

JoVE 1731 3/03/2010

Louise E. Sinks, Emmanuel Roussakis, Tatiana V. Esipova, Sergei A. Vinogradov

Department of Biochemistry and Biophysics, University of Pennsylvania

We present principles of oxygen measurements by phosphorescence quenching and review design of porphyrin-based dendritic nanosensors for oxygen imaging in biological systems.

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[¹⁸F]fluorobenzoate ([¹⁸F]SFB)

JoVE 2755 6/28/2011

Shuang Hou*^1,2,3, Duy Linh Phung*^1,2,3, Wei-Yu Lin^1,2,3, Ming-wei Wang⁴, Kan Liu⁵, Clifton Kwang-Fu Shen^1,2,3

¹Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, ²Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, ³California NanoSystems Institute, University of California at Los Angeles, ⁴Nuclear Medicine, PET Center, Shanghai Medical Collegea, Fudan University, ⁵Electronics and Information Engineering, College of Electronics and Information Engineering, Wuhan Textile University

A facile, one-pot synthesis of N-succinimidyl-4-[¹⁸F]fluorobenzoate ([¹⁸F]SFB) was developed based on a non-aqueous, three-step radiochemical process. Using microwave heating, the entire procedure can be completed in less than 30 min, or 60 min with further purification by preparative HPLC. The decay-corrected radiochemical yields (RCYs) were 35-5% (n > 30).

Synthesis of Nine-atom Deltahedral Zintl Ions of Germanium and their Functionalization with Organic Groups

JoVE 3532 2/11/2012

Miriam M. Gillett-Kunnath, Slavi C. Sevov

Department of Chemistry and Biochemistry, University of Notre Dame

We present the high-temperature synthesis of intermetallic precursors K₄Ge₉, their dissolution in ethylenediamine to form Ge₉^4- deltahedral Zintl ions, and the reaction of the clusters with alkynes to form organo-Zintl ions. The latter are characterized by electrospray mass spectrometry in solutions and by single-crystal X-ray diffraction in the solid state.

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

JoVE 2910 6/13/2011

Aya D. Pusic*¹, Yelena Y. Grinberg*¹, Heidi M. Mitchell², Richard P. Kraig¹

¹Department of Neurology and Committee on Neurobiology, The University of Chicago Medical Center, ²Department of Neurology, The University of Chicago Medical Center

Migraine and its transformation to chronic migraine are immense healthcare burdens in need of improved treatment options. We seek to define how neural immune signaling modulates the susceptibility to migraine, modeled in vitro using spreading depression in hippocampal slice cultures, as a means to develop novel therapeutic targets.