The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

Recommend to Librarian

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by section
General
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
 
 
 JoVE Clinical and Translational Medicine

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy


JoVE 4218 8/14/2012

1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

 JoVE Clinical and Translational Medicine

Spheroid Assay to Measure TGF-β-induced Invasion


JoVE 3337 11/16/2011

Department of Molecular Cell Biology and Centre for Biomedial Genetics, Leiden University Medical Centre

An assay to quantitatively measure Transforming Growth Factor (TGF)-β-induced invasion in 3-dimensional collagen gels is described. This assay takes advantage of the MCF10A series of cell lines, which represent different stages of breast cancer development. This method can be adopted to be used with other cell lines and might be used to investigate other potential activators or inhibitors of invasion.

 JoVE Clinical and Translational Medicine

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis


JoVE 3888 2/17/2012

Department of Cell Biology, Harvard Medical School

The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers. This assay models the early steps of ovarian cancer metastasis.

 JoVE Clinical and Translational Medicine

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression


JoVE 4206 8/28/2012

1Department of Preventive Medicine, University of Southern California, 2Institute for Women's Health, University College London

We describe methodologies for establishing in vitro heterotypic three-dimensional models comprising ovarian fibroblasts and normal ovarian surface or ovarian cancer epithelial cells. We discuss the use of these models to study stromal-epithelial interactions that occur during ovarian cancer development.

 JoVE General

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture


JoVE 3760 4/30/2012

Molecular Oncology Research Institute, Department of Medicine, Tufts Medical Center

A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.

 JoVE Bioengineering

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels


JoVE 50081 12/06/2012

Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis

The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.

 JoVE Bioengineering

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro


JoVE 2425 11/20/2011

Department of Chemical Engineering, University Of Massachusetts Amherst

We present the procedure for fabrication and operation of a microfluidic device that recreates heterogeneous tumor microenvironments in vitro. The variability in apoptosis within tumor tissue was quantified using fluorescent stains and the effective diffusion coefficient of the chemotherapeutic drug doxorubicin into tumor tissue was evaluated.

 JoVE Bioengineering

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria


JoVE 50474 5/08/2013

1Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, 2Clinical Research Laboratory, 81st Medical Group, Keesler Air Force Base

Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.

 JoVE Bioengineering

Measurement of Aggregate Cohesion by Tissue Surface Tensiometry


JoVE 2739 4/08/2011

Department of Surgery, UMDNJ-Robert Wood Johnson Medical School

We describe a method of measuring binding energy, expressible as tissue surface tension, between cells within 3D tissue-like aggregates. Differences in tissue surface tension have been demonstrated to correlate with invasiveness of lung, muscle, and brain tumors, and are fundamental determinants of establishing spatial relationships between different cell types.

 JoVE Clinical and Translational Medicine

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice


JoVE 2644 8/04/2011

1Interdepartmental Program in Translational Biology & Molecular Medicine, Baylor College of Medicine, 2Texas Children's Cancer Center, Baylor College of Medicine, 3Department of Pediatrics, Baylor College of Medicine, 4Department of Pathology, The Methodist Hospital Research Institute, 5Department of Ophthalmology, Retinoblastoma Center of Houston, 6Baylor College of Medicine, Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy, Baylor College of Medicine

A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.

 JoVE General

In vivo-like Organotypic Murine Retinal Wholemount Culture


JoVE 1634 1/11/2010

Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen

This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.

 JoVE Clinical and Translational Medicine

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain


JoVE 4204 8/28/2012

1Department of Surgery, University of Texas Medical Branch, 2Department of Neuroscience and Cell Biology, University of Texas Medical Branch

A method for large-scale purification of the APP intracellular domain (AICD) is described. We also describe methodology to induce in vitro AICD aggregation and visualization by atomic force microscopy. The methods described are useful for biochemical/structural characterization of the AICD and the effects of molecular chaperones on its aggregation.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Bioengineering

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering


JoVE 3366 11/25/2011

Biomedical Engineering Department, Worcester Polytechnic Institute

This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.

Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth


JoVE 2914 8/11/2011

1Department of Biology, University of Haifa, 2Transgenic Oncogenesis and Genomics Section, Laboratory of Cancer Biology and Genetics, National Cancer Institute

A modified 3-D in vitro system is presented in which growth characteristics of several tumor cell lines in reconstituted basement membrane correlate with the dormant or proliferative behavior of the tumor cells at a metastatic secondary site in vivo.

Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

In vitro Organoid Culture of Primary Mouse Colon Tumors


JoVE 50210 5/17/2013

1Department of Molecular & Integrative Physiology, University of Michigan, 2Department of Internal Medicine, Division of Gastroenterology, University of Michigan

A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.

Results below contain some, but not all of your search terms.
 JoVE General

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells


JoVE 4254 10/11/2012

Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

More Results...
Waiting
simple hit counter