1Great Lakes Bioenergy Research Center, Michigan State University (MSU), 2Great Lakes Bioenergy Research Center and DOE-Plant Research Lab, Michigan State University (MSU)
Plant biomass is a major carbon-neutral renewable resource that could be used for the production of biofuels. Plant biomass consists mainly of cell walls, a structurally complex composite material termed lignocellulosics. Here we describe a protocol for a comprehensive analysis of the content and composition of wall derived carbohydrates.
Published March 12, 2010. Keywords: Plant Biology, cell walls, polysaccharide, cellulose, hemicellulose, sugar composition, GC-MS
JoVE Immunology and Infection
1Undergraduate Program, Rice University, 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center, 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 4University of Texas Graduate School of Biological Sciences at Houston
A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.
Published April 16, 2013. Keywords: Immunology, Biochemistry, Molecular Biology, Cellular Biology, Structural Biology, Medicine, Genetics, Proteomics, Proteins, Glycomics, Functional glycomics, glycosphingolipids, GSLs, antigens, natural killer T cells, MALDI-TOF mass spectrometry, LTQ ion trap mass spectrometer, mass spectrometry, glycolipids, lipids
1Department of Biology, San Diego State University, 2Scripps Institution of Oceanography, University of California San Diego
Here, we present a comprehensive protocol to assess the organic and inorganic nutrient availability and the abundance and structure of microbial and viral communities in remote marine environments.
Published November 5, 2014. Keywords: Environmental Sciences, dissolved organic carbon, particulate organic matter, nutrients, DAPI, SYBR, microbial metagenomics, viral metagenomics, marine environment