1Department of Chemistry and Biochemistry, University of Bern
The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.
Published April 3, 2014. Keywords: Chemistry, Nucleic acid analogues, Bioorganic Chemistry, PCR, primer extension reactions, organic synthesis, PAGE, HPLC, nucleoside triphosphates
1Scripps Florida, The Scripps Research Institute
Peptide tertiary amides (PTAs) are a superfamily of peptidomimetics that include but are not limited to peptides, peptoids and N-methylated peptides. Here we describe a synthetic method which combines both split-and-pool and sub-monomer strategies to synthesize a one-bead one-compound library of PTAs.
Published June 20, 2014. Keywords: Chemistry, Split-and-pool synthesis, peptide tertiary amide, PTA, peptoid, high-throughput screening, combinatorial library, solid phase, triphosgene (BTC), one-bead one-compound, OBOC
1Department of Chemistry, Northwestern University, 2Department of Chemistry, Warsaw University of Technology, 3Department of Chemistry, Faculty of Science, King Abdulaziz University
Synthesis, activation, and characterization of intentionally designed metal-organic framework materials is challenging, especially when building blocks are incompatible or unwanted polymorphs are thermodynamically favored over desired forms. We describe how applications of solvent-assisted linker exchange, powder X-ray diffraction in capillaries and activation via supercritical CO2 drying, can address some of these challenges.
Published September 5, 2014. Keywords: Chemistry, Metal-organic frameworks, porous coordination polymers, supercritical CO2 activation, crystallography, solvothermal, sorption, solvent-assisted linker exchange
1Department of Chemistry, Massachusetts Institute of Technology
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
Published October 10, 2013. Keywords: Chemistry, Chemical Engineering, Nanoparticles, Polymers, Drug Delivery Systems, Polymerization, polymers, Biomedical and Dental Materials, brush first, polyethylene glycol, photodegradable, ring opening metathesis polymerization, brush polymer, star polymer, drug delivery, gel permeation chromatography, arm first, core functional, photocleavable
1Department of Chemical Engineering, UC Berkeley, 2Department of Materials Science and Engineering, UC Berkeley, 3Department of Chemistry, UC Berkeley, 4Materials Sciences Division, Lawrence Berkeley National Laboratory, 5Department of Chemistry, University of Chicago, 6Center for Nanoscale Materials, Argonne National Laboratory
A protocol for the seeded synthesis of rod-shaped and tetrapod-shaped multicomponent nanostructures consisting of CdS and CdSe is presented.
Published December 11, 2013. Keywords: Chemistry, nanostructures, synthesis, nanocrystals, seeded rods, tetrapods, nanoheterostructures
1Center for Advanced Microstructures and Devices (CAMD), Louisiana State University, 2Center for Atomic-Level Catalyst Design, Cain Department of Chemical Engineering, Louisiana State University, 3Department of Biological and Agricultural Engineering, Louisiana State University, 4Argonne National Laboratory
Millifluidic devices are utilized for controlled synthesis of nanomaterials, time-resolved analysis of reaction mechanisms and continuous flow catalysis.
Published November 27, 2013. Keywords: Bioengineering, Millifluidics, Millifluidic Device, Time-resolved Kinetics, Synthesis, Catalysis, Nanomaterials, Lab-on-a-Chip
1Max von Pettenkofer Institute, 2Department of Medicine, University of Cambridge, 3Institute for Informatics, Ludwig-Maximilians-University Munich
Total cellular RNA provides a poor template for studying short-term changes in RNA synthesis and decay as well as the kinetics of RNA processing. Here, we describe metabolic labeling of newly transcribed RNA with 4-thiouridine followed by thiol-specific biotinylation and purification of newly transcribed RNA allowing to overcome these limitations.
Published August 8, 2013. Keywords: Genetics, Cellular Biology, Molecular Biology, Microbiology, Biochemistry, Eukaryota, Investigative Techniques, Biological Phenomena, Gene expression profiling, RNA synthesis, RNA processing, RNA decay, 4-thiouridine, 4sU-tagging, microarray analysis, RNA-seq, RNA, DNA, PCR, sequencing
1Department of Chemistry, University of Pittsburgh
Microwave-assisted intramolecular dehydrogenative Diels-Alder (DA) reactions provide concise access to functionalized cyclopenta[b]naphthalene building blocks. The utility of this methodology is demonstrated by one-step conversion of the dehydrogenative DA cycloadducts into novel solvatochromic fluorescent dyes via Buchwald-Hartwig palladium-catalyzed cross-coupling reactions.
Published April 1, 2013. Keywords: Chemistry, Chemical Engineering, Physical Chemistry, Microwave-assisted synthesis, dehydrogenative Diels-Alder reactions, naphthalenes, fluorescent dyes, solvatochromism, catalyst
1Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK
We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.
Published May 13, 2014. Keywords: Cellular Biology, Cancer, RNA synthesis, Hypoxia, Microscopy, Click-iT, Open Microscopy Environment, OMERO
1Department of Chemistry and Biochemistry, University of Notre Dame
We present the high-temperature synthesis of intermetallic precursors K4Ge9, their dissolution in ethylenediamine to form Ge94- deltahedral Zintl ions, and the reaction of the clusters with alkynes to form organo-Zintl ions. The latter are characterized by electrospray mass spectrometry in solutions and by single-crystal X-ray diffraction in the solid state.
Published February 11, 2012. Keywords: Biochemistry, Zintl ions, deltahedral clusters, germanium, intermetallics, alkali metals