PTMScan - Proteomics of Post-translational Modifications - ADVERTISEMENT

JoVE 2849 8/15/2011

Jeffrey C. Silva, Charles L. Farnsworth, Xiaoying Jia, Matthew P. Stokes, Albrecht Moritz, Ailan Guo, Roberto D. Polakiewicz, Michael J. Comb

Site Discovery Group and PhosphoScan Services Group, Cell Signaling Technology, Inc.

PTMScan was developed at Cell Signaling Technology (CST) to quantitatively profile post-translational modifications (PTMs) such as phosphorylation, ubiquitination, and acetylation. Post-translationally modified peptides are isolated with motif antibodies and identified and quantified by LC tandem mass spectrometry. Using PTMScan, CST scientists have identified 10s of thousands of novel PTM sites.