Jove Lab Bio

Lab 10: Cell Division — Materials List

Preparation for 10 Students

• Diluted DAPI (1 µl in 10 mL PBS)
  2.5 mL
• Hydrochloric Acid (HCl), 1 N
  5 mL
• Nocodazole (5 mg/mL)
  2.5 mL
• Test tube rack
  5
• Microcentrifuge tubes (3-5)
  5
• Automated pipettes (1 µL-1mL)
  5
• Glass slides (1 case)
  5
• Glass cover slips (1 case)
  5
• Distilled water (100 mL)
  5
• Spray bottle (70% ethanol)
  5
• Razor blade
  5
• Onions with fresh roots
  Dependent on the lab size
• Heat block with thermometer
  Dependent on the lab size
• Fluorescence microscope
  Dependent on the lab size