1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production
Published March 3, 2015. Keywords: This Month in JoVE,
1Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, 2High Throughput Screening Core Facility, Centre for Integrative Biology, University of Trento
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
Published March 3, 2015. Keywords: Cellular Biology, Post-transcriptional control, 3' UTR, high-throughput screening, cell-based assay, reporter, luciferase, small molecules
1Neurobiology and Behavior, University of California, Irvine, 2Physiology and Biophysics, University of California, Irvine
Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.
Published March 3, 2015. Keywords: Cellular Biology, Calcium, imaging, total internal reflection microscopy, algorithm, automation, fluorescence
1Département de Biologie, Institut de biologie intégrative et des systémes & PROTEO, Université Laval
Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).
Published March 3, 2015. Keywords: Cellular Biology, Protein-protein interaction (PPI); high-throughput screening; yeast; protein-fragment complementation assay (PCA); dihydrofolate reductase (DHFR); high-density arrays; systems biology; biological networks
1School of Psychological Sciences, Tel-Aviv University, 2School of Behavioral Sciences, Academic College of Tel Aviv-Yaffo, 3Department of Education and Psychology, The Open University of Israel, 4School of Health and Life Sciences, Hadassah Academic College
This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair.
Published March 2, 2015. Keywords: Behavior, Depression, forced swim test, FST, mouse, rat, animal model, behavioral neuroscience, antidepressants, SSRI
1Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia
In this paper, an experimental framework to perform closed-loop experiments is presented, in which information processing (i.e., coding and decoding) and learning of neuronal assemblies are studied during the continuous interaction with a robotic body.
Published March 2, 2015. Keywords: Neuroscience, Micro Electrode Arrays (MEA), in vitro cultures, coding, decoding, tetanic stimulation, spike, burst
1Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Turin, 2Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, 3Bracco Research Center, Bracco Imaging SpA
The present work describes a new protocol to perform non-invasive high-frequency ultrasound and photoacoustic based imaging on rat brain, to efficiently visualize deep subcortical regions and their vascular patterns by directing signals on skull foramina naturally present on animal cranium.
Published March 2, 2015. Keywords: Neuroscience, Photoacoustics, High-frequency ultrasounds, Brain imaging, Cerebral hemodynamics, Non-invasive imaging, Small animal, Neuroimaging
1Department of Plastic and Hand Surgery, University of Freiburg Medical Centre
The use of the masseteric nerve as donor nerve represents a single-stage alternative to the criterion standard two-stage procedure of cross-facial nerve grafting and free muscle transfer in facial paralysis. We provide a detailed description to safely perform this technique with a gracilis muscle transfer and discuss indications and limitations.
Published March 1, 2015. Keywords: Medicine, microsurgery, free microvascular tissue transfer, face, head, head and neck surgery, facial paralysis
1Department of Otolaryngology - Head and Neck Surgery, University of Missouri, 2Department of Communication Science and Disorders, University of Missouri, 3Department of Medicine, University of Missouri
This study successfully adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating translational dysphagia research.
Published March 1, 2015. Keywords: Medicine, mouse, murine, rodent, swallowing, deglutition, dysphagia, videofluoroscopy, radiation, iohexol, barium, palatability, taste, translational, disease models
1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.
A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.
Published February 28, 2015. Keywords: Molecular Biology, Aptamer, structure-switching, SELEX, small molecule, cortisol, next generation sequencing, gold nanoparticle, assay