1Radiology, University of Alabama at Birmingham, 2Comprehensive Cancer Center, University of Alabama at Birmingham, 3Radiation Oncology, University of Alabama at Birmingham
The goal of this protocol is to apply dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for orthotopic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy.
Published April 18, 2015. Keywords: Medicine, Imaging, Cancer, Pancreas, Mouse, Xenograft, DCE-MRI
1Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics, Leiden University Medical Center, 2Department of Orthopedic Surgery, Academic Medical Center, 3Department of Plastic, Reconstructive and Hand Surgery, Academic Medical Center, 4Department of Orthopedic Surgery, Mayo Clinic, 5Department of Biochemistry and Molecular Biology, Mayo Clinic, 6Department of Dermatology, Leiden University Medical Center
Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.
Published April 18, 2015. Keywords: Medicine, Fibrosis, Dupuytren's disease, TGFβ, 3D culture system, Compound screen, Extracellular matrix
1Department of Pathology and Laboratory Medicine, Faculty of Medicine, The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory
This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.
Published April 17, 2015. Keywords: Environmental Sciences, environmental samples, next-generation sequencing, DNA libraries, Ranger Technology, gel size selection, metagenomics, water samples
1Department of Mechanical Engineering, University of British Columbia
Two experimental devices for examining liquid jet impingement on a high-speed moving surface are described: an air cannon device and a spinning disk device. The apparatuses are used to determine optimal approaches to the application of liquid friction modifier (LFM) onto rail tracks for top-of-rail friction control.
Published April 17, 2015. Keywords: Engineering, Liquid jet impingement, high-speed moving surface, spray nozzle, liquid friction modifier (LFM), air cannon, spinning disk, rail track lubrication, fluid mechanics
1Agricultural Research Service, United States Department of Agriculture
This protocol was developed to screen a large germplasm collection of the leafy vegetable lettuce (Lactuca sativa L.) for drought-tolerance, in order to identify a small candidate pool of lettuce for use in physiological, molecular, and genetic studies to identify underlying drought-tolerance traits along with breeding programs.
Published April 17, 2015. Keywords: Environmental Sciences, Lettuce, Lactuca sativa, drought, water-stress, abiotic-stress, relative water content
1Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart
This movie shows how an atmospheric plasma torch can be ignited by microwaves with no additional igniters and provides a stable and continuous plasma operation suitable for plenty of applications.
Published April 16, 2015. Keywords: Engineering, atmospheric pressure plasma, microwave plasma, plasma ignition, resonator structure, coaxial resonator, cylindrical resonator, plasma torch, stable plasma operation, continuous plasma operation, high speed camera
JoVE Immunology and Infection
1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science
Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.
Published April 16, 2015. Keywords: Immunology, Naïve CD4+ T cell, T helper cell, Th1, Th2, Th17, Treg
JoVE Immunology and Infection
1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University
In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.
Published April 16, 2015. Keywords: Immunology, Neutrophil, neutrophil extracellular traps, inflammation, bacteria, infection, cancer, metastasis, adhesion
1Department of Neurosurgery, Columbia University Medical Center, New York Presbyterian Hospital, 2Department of Neurology, Columbia University Medical Center, New York Presbyterian Hospital, 3Columbia University Medical Center, New York Presbyterian Hospital, 4School of Medicine, King's College London
Stereotactic Electroencephalography (SEEG) is an operative technique used in epilepsy surgery to help localize seizure foci. It also affords a unique opportunity to investigate brain function. Here we describe how SEEG can be used to investigate cognitive processes in human subjects.
Published April 15, 2015. Keywords: Neuroscience, epilepsy, stereotactic electroencephalography, anterior cingulate cortex, local field potential, electrode placement
1Department of Human and Molecular Genetics, Virginia Commonwealth University, 2VCU Alcohol Research Center, Virginia Commonwealth University
Straightforward assays for measuring ethanol sensitivity and rapid tolerance in Drosophila facilitate the use of this model organism for investigating these important ethanol-related behaviors. Here, a relatively simple, scalable assay for measuring ethanol sensitivity and rapid tolerance in flies is described.
Published April 15, 2015. Keywords: Neuroscience, ethanol, alcohol, behavior, sensitivity, Drosophila, fruit fly, assay