1Radiological Protection Research and Instrumentation, Canadian Nuclear Laboratories
A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.
Published July 3, 2015. Keywords: Molecular Biology, DNA damage, DNA double-strand breaks, DNA repair, γ-H2AX, low dose radiation, tritium, β--radiation, γ-radiation, chronic exposure, flow cytometry, in vivo, mouse model
1Department of Physics, The Ohio State University, 2Department of Chemistry, The Ohio State University
We present the synthesis of the organic-based ferrimagnet vanadium tetracyanoethylene (V[TCNE]x, x~2) via low temperature chemical vapor deposition (CVD). This optimized recipe yields an increase in Curie temperature from 400 K to over 600 K and a dramatic improvement in magnetic resonance properties.
Published July 3, 2015. Keywords: Chemistry, organic-based magnet, thin film, room temperature, spintronics, magnetism, chemical vapor deposition
1Department of Anesthesiology and Perioperative Care, University of California Irvine, 2Department of Biomedical and Molecular Sciences, Queen's University, 3Department of Pharmacology, University of California Irvine
Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.
Published July 3, 2015. Keywords: Molecular Biology, Trafficking, Receptor, G-protein coupled receptor, Internalization, Degradation, Recycling, Colocalization, Immunocytochemistry, Immunohistochemistry, Microscopy
1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production
Published July 2, 2015. Keywords: This Month in JoVE
1Institute of Agro-environmental and Forest Biology (IBAF), National Research Council (CNR), Italy
A robust and flexible approach to confirm herbicide resistance in weed populations is presented. This protocol allows the herbicide resistance levels to be inferred and applied to a wide range of weed species and herbicides with minor adaptations.
Published July 2, 2015. Keywords: Environmental Sciences, Weed science, resistant biotypes, monitoring, seed germination, weed control, herbicide efficacy, herbicide treatment, resistance level.
JoVE Developmental Biology
1Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo
We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.
Published July 2, 2015. Keywords: Developmental Biology, Pluripotent stem cells, encapsulation, alginate, suspension culture, co-axial nozzle, stem cell niche
1Department of Plant Physiology and Biophysics, University of Würzburg, 2EPG Systems, Wageningen, The Netherlands
Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.
Published July 2, 2015. Keywords: Environmental Sciences, Electrophysiology, plant physiology, phloem, electrical penetration graph, sieve element, intracellular recording, electrical signaling, aphid, Arabidopsis, ion channels, electrode, insect-plant interactions
1Department of Didactics, Université du Québec à Montréal, 2Department of IT and Tech3Lab, HEC Montreal, 3Department of Marketing and Tech3Lab, HEC Montreal, 4Department of Specialized Education, Université du Québec à Montréal
This paper aims to describe the techniques involved in the collection and synchronization of the multiple dimensions (behavioral, affective and cognitive) of learners’ engagement during a task.
Published July 1, 2015. Keywords: Behavior, Measurement of engagement, learning, neurophysiology, electroencephalography, signal synchronization, electrodermal activity, automatic facial emotion recognition, emotional valence, arousal
1Center for Molecular & Behavioral Neuroscience, Rutgers University, State University of New Jersey, Newark, 2Department of Audiology, University of the Pacific, 3Department of Otolaryngology, Head & Neck Surgery, Stanford University
High-density electroencephalography (dEEG) is being used increasingly to study brain development and plasticity in the early years of life. Here we present an application of sophisticated analysis techniques that builds on traditional EEG recording to understand the oscillatory dynamics of rapid auditory processing in the infant brain.
Published July 1, 2015. Keywords: Behavior, Infant, Infant Brain, Human Development, Auditory Development, Oscillations, Brain Oscillations, Theta, Electroencephalogram, Child Development, Event-related Potentials, Source Localization, Auditory Cortex
1Department of Family Medicine, The Ohio State University College of Medicine, 2Critical Care Nursing, Wexner Medical Center, 3Department of Psychiatry, Stress, Trauma, and Resilience (STAR) Program, The Ohio State University College of Medicine
The Mindfulness in Motion (MIM) protocol offers a pragmatic Mindfulness Based Intervention (MBI) on-site, for persons working in chronically high-stress work environments that significantly increases resiliency and work engagement. The protocol has proven feasible, beneficial, and is easily adaptable to other high-stress workplaces.
Published July 1, 2015. Keywords: Behavior, Mindfulness, resiliency, work-engagement, stress-reduction, workplace, non-reactivity, Intensive-care, chronic stress, work environment