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  JoVE Immunology and Infection

  
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  JoVE Applied Physics

  
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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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 JoVE Clinical and Translational Medicine

A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

1MRC Centre for Inflammation Research, University of Edinburgh


JoVE 52559

The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.

 JoVE Biology

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

1Department of Molecular Genetics, The Ohio State University


JoVE 52030

Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

 JoVE Biology

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

1Department of Biology, Case Western Reserve University


JoVE 52189

Here, we present a protocol to transform flax using Agrobacterium-mediated plant transformation via floral-dip. This protocol is simple to perform and inexpensive, yet yields a higher transformation rate than the current available methods for flax transformation.

 JoVE Immunology and Infection

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

1Department of Molecular Genetics and Microbiology, Stony Brook University


JoVE 52432

In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.

 JoVE Biology

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

1Department of Plant Sciences, University of Oxford, 2School of Education of Vitoria-Gasteiz, University of the Basque Country (UPV/EHU), 3Biosciences, University of Exeter


JoVE 52113

This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

 JoVE Neuroscience

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg


JoVE 52241

We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.

 JoVE Behavior

Disrupting Reconsolidation of Fear Memory in Humans by a Noradrenergic β-Blocker

1Department of Clinical Psychology, University of Amsterdam


JoVE 52151

Disrupting reconsolidation is a promising approach to dampen the behavioral expression of fear memory in patients with anxiety disorders or posttraumatic stress disorder. In a series of human fear conditioning studies we showed that disrupting reconsolidation by the noradrenergic β-blocker propranolol is very effective in erasing conditioned fear responding.

 JoVE Applied Physics

Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis

1Materials Science and Engineering, School of Engineering, University of California Merced


JoVE 51384

A new computational system featuring GPU-accelerated molecular dynamics simulation and 3D/VR visualization, analysis and manipulation of nanostructures has been implemented, representing a novel approach to advance materials research and promote innovative investigation and alternative methods to learn about material structures with dimensions invisible to the human eye.

 JoVE Clinical and Translational Medicine

MRI Mapping of Cerebrovascular Reactivity via Gas Inhalation Challenges

1Advanced Imaging Research Center, University of Texas Southwestern Medical Center, 2Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine


JoVE 52306

Non-invasive imaging of the brain vasculature’s ability to dilate or constrict may allow a better understanding of cerebrovascular pathophysiology in various neurological diseases. The present report describes a reproducible and patient-comfortable protocol to perform vascular reactivity imaging in humans using magnetic resonance imaging (MRI).

 JoVE Clinical and Translational Medicine

Mouse Pneumonectomy Model of Compensatory Lung Growth

1Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center


JoVE 52294

Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.

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