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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Essentials of Biology 1

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 JoVE Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

1Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, 2High Throughput Screening Core Facility, Centre for Integrative Biology, University of Trento


JoVE 52568

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

 JoVE Biology

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

1Neurobiology and Behavior, University of California, Irvine, 2Physiology and Biophysics, University of California, Irvine


JoVE 52516

Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.

 JoVE Biology

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

1Département de Biologie, Institut de biologie intégrative et des systémes & PROTEO, Université Laval


JoVE 52255

Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

 JoVE Behavior

The Forced Swim Test as a Model of Depressive-like Behavior

1School of Psychological Sciences, Tel-Aviv University, 2School of Behavioral Sciences, Academic College of Tel Aviv-Yaffo, 3Department of Education and Psychology, The Open University of Israel, 4School of Health and Life Sciences, Hadassah Academic College


JoVE 52587

This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair.

 JoVE Neuroscience

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

1Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia


JoVE 52341

In this paper, an experimental framework to perform closed-loop experiments is presented, in which information processing (i.e., coding and decoding) and learning of neuronal assemblies are studied during the continuous interaction with a robotic body.

 JoVE Neuroscience

Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging

1Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Turin, 2Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, 3Bracco Research Center, Bracco Imaging SpA


JoVE 52162

The present work describes a new protocol to perform non-invasive high-frequency ultrasound and photoacoustic based imaging on rat brain, to efficiently visualize deep subcortical regions and their vascular patterns by directing signals on skull foramina naturally present on animal cranium.

 JoVE Medicine

Single-stage Dynamic Reanimation of the Smile in Irreversible Facial Paralysis by Free Functional Muscle Transfer

1Department of Plastic and Hand Surgery, University of Freiburg Medical Centre


JoVE 52386

The use of the masseteric nerve as donor nerve represents a single-stage alternative to the criterion standard two-stage procedure of cross-facial nerve grafting and free muscle transfer in facial paralysis. We provide a detailed description to safely perform this technique with a gracilis muscle transfer and discuss indications and limitations.

 JoVE Medicine

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

1Department of Otolaryngology - Head and Neck Surgery, University of Missouri, 2Department of Communication Science and Disorders, University of Missouri, 3Department of Medicine, University of Missouri


JoVE 52319

This study successfully adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating translational dysphagia research.

 JoVE Biology

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.


JoVE 52545

A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

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