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  JoVE Biology

  
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  JoVE Immunology and Infection

  
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  JoVE Medicine

  
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  JoVE Bioengineering

  
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  JoVE Developmental Biology

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 JoVE Immunology and Infection

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

1Department of Biological Sciences, University of Wisconsin Milwaukee


JoVE 53105

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).

 JoVE Medicine

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

1Bloomberg School of Public Health, Environmental Health Sciences, Johns Hopkins University, 2Department of Medicine, Pulmonary Diseases and Critical Care Medicine, University of North Carolina School of Medicine


JoVE 52964

The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.

 JoVE Biology

Repeated Blood Collection for Blood Tests in Adult Zebrafish

1Department of Translational Medical Science, Graduate School of Medicine, Mie University, 2Department of Molecular and Cellular Pharmacology, Pharmacogenomics, and Pharmacoinformatics, Graduate School of Medicine, Mie University


JoVE 53272

Repeated blood sampling is necessary and important in animal research. We developed a novel, non-lethal and reliable method for repeated blood collection from adult zebrafish, and applied this method to the study of blood biochemistry, including glucose metabolism.

 JoVE Biology

Identification of Kinase-substrate Pairs Using High Throughput Screening

1Children's Hospital of Eastern Ontario Research Institute, 2Sunnybrook Research Institute, University of Toronto, 3Department of Biochemistry, University of Toronto


JoVE 53152

Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.

 JoVE Bioengineering

Fabricating Superhydrophobic Polymeric Materials for Biomedical Applications

1Department of Biomedical Engineering, Boston University, 2Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University


JoVE 53117

Two- and three-dimensional superhydrophobic polymeric materials are prepared by electrospinning or electrospraying biodegradable polymers blended with a lower surface energy polymer of similar composition.

 JoVE Chemistry

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

1School of Pharmacy, Pacific University, 2Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University


JoVE 53047

The goal of this protocol is to describe the preparation and characterization of physically entrapped, poorly water soluble drugs in micellar drug delivery systems composed of amphiphilic block copolymers.

 JoVE Biology

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

1Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School


JoVE 52982

De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.

 JoVE Biology

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

1Department of Cell & Developmental Biology, SUNY Upstate Medical University


JoVE 52949

Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.

 JoVE Engineering

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

1Department of Materials Science and Engineering, University of California Berkeley, 2Materials Science Division, Lawrence Berkeley National Laboratory, 3Advanced Light Source Division, Lawrence Berkeley National Laboratory, 4Department of Chemical and Biomolecular Engineering, University of California Berkeley, 5Environmental Energy Technology Division, Lawrence Berkeley National Laboratory


JoVE 53021

Synchrotron-based hard X-ray microtomography is used to image the electrochemical growth of dendrites from a lithium metal electrode through a solid polymer electrolyte membrane.

 JoVE Engineering

Fabrication and Operation of a Nano-Optical Conveyor Belt

1Electrical Engineering, Stanford University, 2Applied Physics, Stanford University


JoVE 52842

The scalability and resolution of conventional optical manipulation techniques are limited by diffraction. We circumvent the diffraction limit and describe a method of optically transporting nanoparticles across a chip using a gold surface patterned with a path of closely spaced C-shaped plasmonic resonators.

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