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General Laboratory Techniques

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 JoVE Biology

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

1Radiological Protection Research and Instrumentation, Canadian Nuclear Laboratories


JoVE 52912

A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.

 JoVE Chemistry

Chemical Vapor Deposition of an Organic Magnet, Vanadium Tetracyanoethylene

1Department of Physics, The Ohio State University, 2Department of Chemistry, The Ohio State University


JoVE 52891

We present the synthesis of the organic-based ferrimagnet vanadium tetracyanoethylene (V[TCNE]x, x~2) via low temperature chemical vapor deposition (CVD). This optimized recipe yields an increase in Curie temperature from 400 K to over 600 K and a dramatic improvement in magnetic resonance properties.

 JoVE Biology

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

1Department of Anesthesiology and Perioperative Care, University of California Irvine, 2Department of Biomedical and Molecular Sciences, Queen's University, 3Department of Pharmacology, University of California Irvine


JoVE 52824

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

 JoVE Environment

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

1Institute of Agro-environmental and Forest Biology (IBAF), National Research Council (CNR), Italy


JoVE 52923

A robust and flexible approach to confirm herbicide resistance in weed populations is presented. This protocol allows the herbicide resistance levels to be inferred and applied to a wide range of weed species and herbicides with minor adaptations.

 JoVE Developmental Biology

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

1Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo


JoVE 52835

We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

 JoVE Environment

A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

1Department of Plant Physiology and Biophysics, University of Würzburg, 2EPG Systems, Wageningen, The Netherlands


JoVE 52826

Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.

 JoVE Behavior

Assessing the Multiple Dimensions of Engagement to Characterize Learning: A Neurophysiological Perspective

1Department of Didactics, Université du Québec à Montréal, 2Department of IT and Tech3Lab, HEC Montreal, 3Department of Marketing and Tech3Lab, HEC Montreal, 4Department of Specialized Education, Université du Québec à Montréal


JoVE 52627

This paper aims to describe the techniques involved in the collection and synchronization of the multiple dimensions (behavioral, affective and cognitive) of learners’ engagement during a task.

 JoVE Behavior

Infant Auditory Processing and Event-related Brain Oscillations

1Center for Molecular & Behavioral Neuroscience, Rutgers University, State University of New Jersey, Newark, 2Department of Audiology, University of the Pacific, 3Department of Otolaryngology, Head & Neck Surgery, Stanford University


JoVE 52420

High-density electroencephalography (dEEG) is being used increasingly to study brain development and plasticity in the early years of life. Here we present an application of sophisticated analysis techniques that builds on traditional EEG recording to understand the oscillatory dynamics of rapid auditory processing in the infant brain.

 JoVE Behavior

Mindfulness in Motion (MIM): An Onsite Mindfulness Based Intervention (MBI) for Chronically High Stress Work Environments to Increase Resiliency and Work Engagement

1Department of Family Medicine, The Ohio State University College of Medicine, 2Critical Care Nursing, Wexner Medical Center, 3Department of Psychiatry, Stress, Trauma, and Resilience (STAR) Program, The Ohio State University College of Medicine


JoVE 52359

The Mindfulness in Motion (MIM) protocol offers a pragmatic Mindfulness Based Intervention (MBI) on-site, for persons working in chronically high-stress work environments that significantly increases resiliency and work engagement. The protocol has proven feasible, beneficial, and is easily adaptable to other high-stress workplaces.

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