Encyclopedia of Experiments: Biological Techniques
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Transcript
For fluorescence anisotropy-based protein-protein interaction detection, begin with recombinant target proteins tagged with C-terminal tetracysteine motifs in a suitable anisotropy buffer.
Add a fluorophore-containing dye — binding to target proteins via the tetracysteine motif. Dialyze with the anisotropy buffer, removing unbound dye. Transfer the mixture to quartz cuvettes. Measure the absorbance, determining the percentage of successfully-labeled target proteins.
In a fluorescence cuvette, mix the fluorophore-labeled target proteins with unlabeled proteins. Using a spectrofluorometer, measure fluorescence anisotropy.
As the target proteins suspend freely in the buffer, the fluorophores attached to them rotate randomly around their axes due to Brownian motion. Upon excitation with vertically-polarized light of an appropriate wavelength, the fluorophores emit light polarized in the vertical and horizontal planes. The ratio of the fluorescence intensities of the vertical and horizontally polarized emissions — anisotropy — is measured.
When unlabeled proteins bind to the fluorophore-labeled targets, the molecular size of the protein complex increases, reducing its rotational motion. As a result, the emitted light becomes more polarized, with higher emission in the vertical plane than the horizontal plane — increasing anisotropy.
Add increasing concentrations of the unlabeled protein and repeat the anisotropy measurement.
A non-linear increase in anisotropy with increased unlabeled protein addition indicates unlabeled protein binding at multiple non-identical binding sites on the fluorophore-tagged target proteins.
