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Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

Article DOI: 10.3791/60462-v • 12:02 min • August 8th, 2020
August 8th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

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Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).

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Tg(Vtg1:mcherry) Zebrafish Embryos Estrogenic Effects Endocrine Disrupting Compounds EDC Chemicals Chemical Diversity Detection Methods Estrogenic Substances Biomonitor Organisms Bioindicator Organisms Gene Products Molecular Methods Protein Level MRNA Level Animal Sacrifice Animal Protection Laws Alternative Test Systems Cross-genetic Technologies Fluorescent Proteins Biomarker Creation Activation Of Estrogen Sensitive Genes Vtg:1mCherry Transgene Zebrafish Embryos
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