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Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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Summary
Automatic Translation

This protocol demonstrates methods to enable extended in vitro culture of patient-derived xenografts (PDX). One step enhances overall viability of multicellular cluster cultures in 3D hydrogels, through straightforward removal of non-viable single cells. A secondary step demonstrates best practices for PDX culture in a perfused microfluidic platform.

Tags

Ex Vivo 3D Hydrogel Cultures Patient-derived Xenografts Perfused Microfluidic Platform Culture Methods Tumor Samples In Vitro Culture High Throughput Screening High Content Screening In Vivo Cancer Models PDXs Drug Mechanisms Drug Screening Co-cultures Stroma Endothelium Immune Cells Microfluidic Plates Cell Lines Tumor Tissue Sterile PBS
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