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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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Summary
Automatic Translation

In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal tissues.

Tags

Tonsillar Mononuclear Cells Immune Cell Response Mucosal Lymphoid Tissue Ex Vivo Studies Isolation Technique Immune Cell Isolation Tonsil Handling Cell Strainer Culture Dish Sterile Instruments Infectious Agents PBS Solution Tonsil Dissection Tissue Viability Cell Yield Cauterized Tissue Removal
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