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Biology
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Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
 

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

Article DOI: 10.3791/61774-v 13:29 min January 16th, 2021
January 16th, 2021

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Summary

We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana benthamiana. This protocol can be extended to purification and visualization of numerous proteins that utilize column chromatography. Moreover, the protocol is adaptable to the in-person and virtual college teaching laboratory, providing project-based exploration.

Tags

IgG Fusion Proteins Nicotiana Benthamiana Expression Extraction Purification Recombinant Human IgG GFP Fusion Plant-produced Antibodies Column Chromatography Visualization Protein Production Process Soil Peat Pellets Tray Water Seeding Date Growth Chamber Photo Period Relative Humidity
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