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JoVE Journal Biochemistry
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
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Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Article DOI: 10.3791/63729-v • 05:27 min • July 20th, 2022
July 20th, 2022 •
1, 1,2
1Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, 2Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

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Summary
Automatic Translation

This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na+, to study the existence of partially segmented functional CoQ pools.

Tags

Inner Mitochondrial Membrane Na+ Sensitivity Functional CoQ Pools Sodium As A Second Messenger Ubiquinol Pools Electron Transfer Lipid Drops Sample Splitting Labeling Buffer Preparation Temperature Control Cytochrome C Malonate Potassium Chloride Sodium Chloride Rotenone NADH Spectrophotometer Measurement
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