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Photo-Induced Cross-Linking delle proteine ​​non modificati (PICUP) applicata a peptidi amiloidogenico
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Photo-Induced Cross-Linking delle proteine ​​non modificati (PICUP) applicata a peptidi amiloidogenico

Article DOI: 10.3791/1071-v • 08:40 min • January 12th, 2009
January 12th, 2009 •
1, 1, 2,3
1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute, University of California, Los Angeles, 3Department of Neurology, University of California, Los Angeles

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Foto-indotta cross-linking delle proteine ​​non modificato (PICUP) permette la caratterizzazione della distribuzione dimensionale oligomeri in miscele proteiche metastabili. Dimostriamo applicazione di PICUP a tre peptidi rappresentante amiloidogenico il 40 - e 42-residuo forme di β-amiloide, proteina, e la calcitonina, e un peptide di controllo della crescita-fattore di rilascio dell'ormone.

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cross-linking Numero 23 PICUP β-amiloide di proteine oligomero amiloide proteina di montaggio
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