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Foto-Induced bryggbindningen av Oförändrad Proteiner (picup) Tillämpat på Amyloidogenic Peptides
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Foto-Induced bryggbindningen av Oförändrad Proteiner (picup) Tillämpat på Amyloidogenic Peptides

Article DOI: 10.3791/1071-v • 08:40 min • January 12th, 2009
January 12th, 2009 •
1, 1, 2,3
1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute, University of California, Los Angeles, 3Department of Neurology, University of California, Los Angeles

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Foto-inducerad bryggbindningen av omodifierade proteiner (picup) tillåter karakterisering av oligomerer storleksfördelningen i metastabila protein blandningar. Vi visar tillämpning av picup till tre representativa amyloidogenic peptider i 40 - och 42-rester former av amyloid β-protein, och kalcitonin, och en kontroll peptid tillväxt-hormone releasing faktor.

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bryggbindningen picup amyloid β-protein oligomerer amyloid protein montering
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