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Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Article DOI: 10.3791/1086-v • 13:51 min • February 18th, 2009
February 18th, 2009 •
1, 1, 1, 1, 1
1Department of Marine Sciences, University of Georgia (UGA)

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Summary
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We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.

Tags

Gene Expression Marine Microbial Communities Environmental Transcriptomics Metatranscriptomics MRNA Sequencing Prokaryotic MRNA Poly(A) Tails Eukaryotic Messages Clone Libraries Random Primers CDNA Synthesis Linear Amplification In Vitro Transcription
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