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Maus Adrenal Chromaffin Zellisolation
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Maus Adrenal Chromaffin Zellisolation

Article DOI: 10.3791/129-v • 18:30 min • January 5th, 2007
January 5th, 2007 •
1, 2,3, 4, 1, 2,3
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of Southern California, Keck School of Medicine, 3Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, 4Department of Developmental and Cell Biology, University of California, Irvine (UCI)

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Nebennierenmarks chromaffinen Zellkultur-Systeme sind äußerst nützlich für die Untersuchung der Anregungs-Sekretion Kopplung in einem in vitro-Einstellung. Dieses Protokoll stellt das Verfahren verwendet werden, um den Nebennieren zu sezieren und dann isolieren medulläre Region durch Abstreifen der Nebennierenrinde. Die Verdauung der Medulla in einzelne chromaffinen Zellen wird dann demonstriert.

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Developmental Biology Ausgabe 2 Neuroscience Maus- Nebennieren-
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