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Primaire Cultuur van volwassen ratten Heart myocyten
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Primaire Cultuur van volwassen ratten Heart myocyten

Article DOI: 10.3791/1308-v • 11:44 min • June 16th, 2009
June 16th, 2009 •
1, 1,2
1Department of Physiology and Cellular Biophysics, Columbia University, 2Department of Pharmacology, Columbia University

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In dit artikel beschrijven we een typische manier om te isoleren en cultuur volwassen ratten hart myocyten. Collagenase en protease worden gebruikt voor het verteren en te isoleren single myocyten. Myocyten gekweekte volgen van dit protocol voldoen aan de meeste eisen experiment.

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Cellular Biology Rat hart isolatie primaire cultuur cardiologie
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