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Journal / Biology / 一个外源凝集素的高效液相色谱法从复杂生物样品，以丰富的选择性糖基化肽…
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Biology

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一个外源凝集素的高效液相色谱法从复杂生物样品，以丰富的选择性糖基化肽
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一个外源凝集素的高效液相色谱法从复杂生物样品，以丰富的选择性糖基化肽

Article DOI: 10.3791/1398-v • 20:24 min • October 1st, 2009
October 1st, 2009 •
1, 2, 1, 1, 1, 2, 1, 1, 1, 3, 2, 1, 1
1Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco - UCSF, 2Buck Institute for Age Research, 3Department of Chemistry, Purdue University

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凝集素结合POROS珠被雇用的高效液相色谱法。糖肽类标准担任阳性和阴性对照。 MARS的14枯竭，胰蛋白酶消化人体血浆层析和流过（FT）和约束分数ESI-LC-MS/MS分析收集。糖肽的富集在绑定的分数相比，FT。

Tags

基本协议，第32期，凝集素，色谱仪，糖肽，糖蛋白，生物标志物的发现
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