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 JoVE Immunology and Infection

Yüksek Saflaştırılmış Alınması Toxoplasma gondii Süreksiz Sezyum Klorür Gradient ookistleri

1, 2, 3, 2,4

1Dynamac, Inc., 2Department of Biological Sciences, University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory, US Environmental Protection Agency

 

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Cite this Article: Yüksek Saflaştırılmış Alınması Toxoplasma gondii Süreksiz Sezyum Klorür Gradient ookistleri

Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

Disclosures: Yüksek Saflaştırılmış Alınması Toxoplasma gondii Süreksiz Sezyum Klorür Gradient ookistleri

References: Yüksek Saflaştırılmış Alınması Toxoplasma gondii Süreksiz Sezyum Klorür Gradient ookistleri

  1. Dubey, J.P. et al. Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers. J. Parasitol. 91, 1082-93 (2005).
  2. Tenter, A.M., Heckeroth, A.R. & Weiss, L.M. Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217-58 (2000).
  3. Bowie, W.R. et al. Outbreak of toxoplasmosis associated with municipal drinking water. Lancet 350, 173-7 (1997).
  4. de Moura, L. et al. Waterborne toxoplasmosis, Brazil, from field to gene. Emerg. Infect. Dis. 12, 326-9 (2006).
  5. Jones, J.L. & Dubey, J.P. Waterborne toxoplasmosis - Recent developments. Exp. Parasitol. doi: 10.1016/j.exppara.2009.03.013 (2009).
  6. Dumetre, A. & Darde, M.L. How to detect Toxoplasma gondii oocysts in environmental samples? FEMS Microbiol. Rev. 27, 651-61 (2003).
  7. Dumetre, A. & Darde, M.L. Purification of Toxoplasma gondii oocysts by cesium chloride gradient. J. Microbiol. Methods 56, 427-30 (2004).
  8. Dubey, J.P. Toxoplasmosis of Animals and Man. 2nd ed. CRC Press, Inc., Boca Raton, FL. In press (2009).

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1 Comment

Good protocol for study of Toxoplasma gondii!

1

Reply

Posted by: Wenchao YanApril 17, 2011, 10:07 AM

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