1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2Department of Pathology, University of California, Irvine (UCI), 3Department of Physiology and Biophysics, University of California, Irvine (UCI)
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Currle, D. S., Hu, J. S., Kolski-Andreaco, A., Monuki, E. S. Culture of Mouse Neural Stem Cell Precursors. J. Vis. Exp. (2), e152, doi:10.3791/152 (2007).
Great advances in our understanding of CNS development and stem cell biology have been made possible by our ability to harvest, isolate and culture embryonic neural stem cells. This video demonstrates the dissection of E12.5 mouse cerebral cortex and the subsequent disaggregation and culturing of embryonic neural stem cells. Many other other similar methods have been successfully employed by other investigators.
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1. Currle, D., Cheng, X., Hsu, C., and Monuki, E. Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelial formation. Development (Cambridge, England) 132(15), 3549-3559 (2005).
2. Flanagan, L., Rebaza, L., Derzic, S., Schwartz, P., and Monuki, E. Regulation of human neural precursor cells by laminin and integrins. J Neurosci Res 83(5), 845-856 (2006).