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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Article DOI: 10.3791/1546-v • 15:29 min • July 10th, 2010
July 10th, 2010 •
1, 1, 1, 1
1Blood Research Institute, BloodCenter of Wisconsin

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Summary
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For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.

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Purification Specific Cell Population Fluorescence Activated Cell Sorting FACS Technique Phenotype Bulk Methods Panning Complement Depletion Magnetic Bead Separation Advantages High Purity Identifying Marker Differential Marker Density Internal Staining Intracellular Protein Expression Genetically Modified Fluorescent Protein Marker Size Granularity Fluorescence Fluorescently-tagged Monoclonal Antibodies Negative Selection Flow Cytometer Sorting Capacity Software
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