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Freezing and Thawing Human Embryonic Stem Cells
 

Freezing and Thawing Human Embryonic Stem Cells

Article DOI: 10.3791/1555-v 08:49 min December 24th, 2009
December 24th, 2009

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Summary

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of freezing and thawing, special care must taken. Here we demonstrate the proper technique for rapidly thawing hES cells from liquid nitrogen stocks, plating them on mouse embryonic feeder cells, and slowly freezing them for long-term storage.

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Freezing Thawing Human Embryonic Stem Cells Technique Isolate Grow Culture Freezing Cells Thawing Cells Expanding Cells Frozen Stock HES Cell Culture Sensitive Stresses Special Care Rapidly Thawing Liquid Nitrogen Stocks Plating Mouse Embryonic Feeder Cells Slowly Freezing Long-term Storage
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