The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
Authors
The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
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Wolfson Centre for Age-Related Diseases, King's College London
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Kaul, A. K., Bateman, J. M. A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila. J. Vis. Exp. (34), e1617, doi:10.3791/1617 (2009).
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解剖果蝇眼睛成虫光盘很简单，但可以利用的时间掌握。关键步骤是确定眼睛的光盘和清扫远离幼虫大脑。眼盘的鉴定是比较容易的，使用这里描述的眼RFP的线。可能修改删除触角光盘或peripodial膜，或离开视叶附以可视化的公关轴突进入大脑。
眼RFP的路线是通过克隆产生特有的一种新型的眼睛， 从2指出的P2基因的第二内含子增强剂的PRED的H -毒刺增强记者载体3成的元素，并以此来建立转基因果蝇。减贫战略的一个特定子集和视锥细胞（图2）。的眼RFP的记者表示。因此，它可以被用于确定是否感兴趣的基因在这些细胞类型中表达。眼RFP的线是根据要求提供。
果蝇眼睛的发展是一个强大的模型研究的基因和分子途径控制神经元的公关1。这里描述的方法和记者可以使用，以进一步了解这一过程。
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这项工作是由伦敦大学国王学院和英国皇家学会。
