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Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
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Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Article DOI: 10.3791/1630-v • 09:23 min • December 21st, 2009
December 21st, 2009 •
1,2, 1, 1,2
1Joint Graduate Program in Cell and Developmental Biology, UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, 2Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey

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Summary
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We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.

Tags

Electron Microscopy Drosophila Embryonic Heart Tube Cell Migration Cell-cell Adhesion Cell Shape Changes Embryonic Development Lumen Visualization Membrane Features Whole Mount Embryos Transmission Electron Microscopy Fixation Embedding Sectioning Late Stage Drosophila Embryos
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