Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
 

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

Article DOI: 10.3791/1669-v 11:33 min December 23rd, 2009
December 23rd, 2009

Chapters

Summary

Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.

Tags

Plasma Membrane Proteins Endocytic Rates Reversible Biotinylation Protein Trafficking Protein Surface Expression Mechanisms Of Endocytosis Direct Endocytic Rate Measurements Labeled Receptor Ligands Transporters Pumps Ion Channels Dopamine Transporter (DAT) Internalization Rates Trafficking Studies
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter