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Split-ubiquitin Baserat Membrane Jäst Två Hybrid (MYT) System
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Split-ubiquitin Baserat Membrane Jäst Två Hybrid (MYT) System: ett kraftfullt verktyg för att identifiera Protein-protein interaktioner

Article DOI: 10.3791/1698-v • 14:04 min • February 1st, 2010
February 1st, 2010 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Biochemistry, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto

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Myt tillåter känslig detektion av transienta och stabil interaktioner mellan proteiner som uttrycks i modellen organismen Saccharomyces cerevisiae. Det har med framgång använts för att studera exogena och jäst integrerade membranproteiner i syfte att identifiera sina samverkande partner i en hög genomströmning sätt.

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Cellbiologi protein-protein växelverkan membran split-ubiquitin jäst bibliotek screening Y2H jäst två-hybrid myt
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