This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
Date Published: 3/04/2010, Issue 37; doi: 10.3791/1717
Keywords: Developmental Biology, Issue 37, adult, zebrafish, organs, dissection, anatomy
Gupta, T., Mullins, M. C. Dissection of Organs from the Adult Zebrafish. J. Vis. Exp. (37), e1717, doi:10.3791/1717 (2010).
Over the last 20 years, the zebrafish has become a powerful model organism for understanding vertebrate development and disease. Although experimental analysis of the embryo and larva
is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking. The organs of the larva undergo significant changes in their overall structure, morphology, and anatomical location during the larval
to adult transition. Externally, the transparent larva develops its characteristic adult striped pigment pattern and paired pelvic fins, while internally, the organs undergo massive growth and remodeling. In addition, the bipotential gonad primordium develops into either testis or ovary. This protocol identifies many of the organs of the adult and demonstrates methods for dissection of the brain, gonads, gastrointestinal system, heart, and kidney of the adult zebrafish. The dissected organs can be used for in situ hybridization, immunohistochemistry, histology, RNA
extraction, protein analysis, and other molecular techniques. This protocol will assist in the broadening of studies in the zebrafish to include the remodeling of larval organs, the morphogenesis of organs specific to the adult and other investigations of the adult organ systems.
Experiments on animals were performed in accordance with the guidelines and regulations set forth by the University of Pennsylvania Institutional Animal Care and Use Committee.
This work was supported by NIH grant R01 HD050901 to MCM and American Cancer Society Postdoctoral Fellowship #PF-05-041-01-DDC to TG.