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Journal / Immunology and Infection / Neutrophiles pièges extracellulaires: Comment…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Neutrophiles pièges extracellulaires
Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
Journal (Immunology and Infection)
Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Journal (Immunology and Infection)
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
 
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Neutrophiles pièges extracellulaires: Comment générer et de les visualiser

Article DOI: 10.3791/1724-v • 14:17 min • February 24th, 2010
February 24th, 2010 •
1, 1, 1,2, 1,2, 2
1Core Facility Microscopy, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

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Neutrophiles pièges extracellulaires (NET) sont un important mécanisme immunitaire inné pour combattre les bactéries pathogènes, champignons et parasites. Nous décrivons ici les méthodes d'isoler granulocytes neutrophiles dans le sang humain et de les activer pour former NET. Nous présentons les techniques de préparation de visualiser NET en microscopie optique et électronique.

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JoVE immunologie No. 36 des neutrophiles des granulocytes neutrophiles Piège extracellulaire NET l'isolement immunomarquage microscopie électronique
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