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Journal / Immunology and Infection / Neutrofielen Extracellulaire Traps: Hoe te…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Neutrofielen Extracellulaire Traps
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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
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Neutrofielen Extracellulaire Traps: Hoe te genereren en te visualiseren

Article DOI: 10.3791/1724-v • 14:17 min • February 24th, 2010
February 24th, 2010 •
1, 1, 1,2, 1,2, 2
1Core Facility Microscopy, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

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Neutrofielen extracellulaire Vallen (NET's) zijn een belangrijk aangeboren immuun mechanisme om pathogene bacteriën, schimmels en parasieten te bestrijden. Hier beschrijven we methoden om neutrofiele granulocyten isoleren uit menselijk bloed en ze te activeren om netten te vormen. We presenteren bereidingstechnieken aan netten in licht-en elektronenmicroscopie te visualiseren.

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Jove Immunologie neutrofielen Granulocyt neutrofielen extracellulaire Trap NET isolatie immunokleuring elektronenmicroscopie
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