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Die Beurteilung Zweidimensionale Kristallisation Trials of Small Membranproteinen für Strukturbiologie Studien Electron Crystallography

Article DOI: 10.3791/1846-v • 09:24 min • October 29th, 2010
October 29th, 2010 •
*1, *1,2, *3, *1, 3, 1,3
1School of Biology, Georgia Institute of Technology, 2Department of Molecular Pharmacology, RWTH Aachen University, 3School of Chemistry and Biochemistry, Georgia Institute of Technology
* These authors contributed equally

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Auswertung zweidimensionaler (2D) Kristallisation Studien für die Bildung geordneter membrane protein-Arrays ist eine höchst kritische und schwierige Aufgabe in Elektronenkristallographie. Hier beschreiben wir unseren Ansatz in Screening und Identifizierung von 2D-Kristallen von überwiegend kleinen Membranproteine ​​im Bereich von 15 - 90kDa.

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Cellular Biology Ausgabe 44 Membranprotein Struktur zweidimensionale Kristallisation Elektronen-Kristallographie Elektronenmikroskopie Screening
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