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Journal / Biology / Hallo-C: eine Methode, um die dreidimensionale…
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Biology

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Hallo-C: eine Methode, um die dreidimensionale Architektur des Genoms Study.

Article DOI: 10.3791/1869-v • 22:27 min • May 6th, 2010
May 6th, 2010 •
*1, *2,3,4,5, *2, 6, 2, 3,6, 1, 2,7,8
1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology, Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, 5Department of Applied Mathematics, Harvard University, 6Department of Physics, Massachusetts Institute of Technology, 7Department of Systems Biology, Harvard Medical School, 8Department of Biology, Massachusetts Institute of Technology
* These authors contributed equally

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Die Hallo-C-Methode ermöglicht unvoreingenommene, genomweite Identifizierung von Chromatin-Wechselwirkungen (1). Hallo-C Paare Ligationssysteme und massiv parallele Sequenzierung. Die resultierenden Daten können verwendet werden, um genomische Architektur auf mehreren Skalen zu studieren: die ersten Ergebnisse identifizierten Features wie Chromosom Gebiete, Segregation von offenen und geschlossenen Chromatin und Chromatin-Struktur an der Megabasen.

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Cellular Biology Ausgabe 39 Chromosome Konformation zu erfassen Chromatin-Struktur Illumina Paired End-Sequenzierung Polymer-Physik.
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