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Primer-Free Selezione aptamero utilizzando una libreria di DNA casuale
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Primer-Free Selezione aptamero utilizzando una libreria di DNA casuale

Article DOI: 10.3791/2039-v • 11:14 min • July 26th, 2010
July 26th, 2010 •
1, 1, 1, 2, 2, 3,4
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University

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Protocolli di SELEX comprendono vari cicli di selezione, ognuno dei quali richiedono la rigenerazione di leganti legato, che a loro volta richiedono sequenze primer fisso fiancheggianti le regioni biblioteca casuale. Queste sequenze di innesco fisso può interferire con il processo di selezione (falsi positivi e negativi). Qui vi presentiamo un primer senza protocollo.

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Biologia Cellulare Numero 41 aptamero selezione S100B panino
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