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Journal / Neuroscience / Ultraschall-gesteuerte Mikroinjektion in der Maus …
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Neuroscience

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JoVE Journal Neuroscience
Ultraschall-gesteuerte Mikroinjektion in der Maus Vorderhirnneuronen In Utero In E9.5
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Ultraschall-gesteuerte Mikroinjektion in der Maus Vorderhirnneuronen In Utero In E9.5

Article DOI: 10.3791/2047-v • 07:48 min • November 13th, 2010
November 13th, 2010 •
1, 1,2
1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs, Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology, Johns Hopkins University School of Medicine

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In utero Überleben Chirurgie in Mäusen ermöglicht die molekulare Manipulation der Genexpression während der Entwicklung. Hier beschreiben wir den Einsatz von Hochfrequenz-Ultraschall-Bildgebung, um die Injektion von retroviralen Vektoren in Gehirn der Maus am Embryonaltag (E) 9,5 führen.

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Neuroscience Ausgabe 45 Hochfrequenz-Ultraschall-Bildgebung Nagetier Überleben Chirurgie intra-ventrikuläre Mikroinjektion retroviralen Vektoren Gen-Transduktion
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