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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Article DOI: 10.3791/2314-v • 13:00 min • January 19th, 2011
January 19th, 2011 •
1, 1, 1
1Department of Biology, University of Waterloo

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Summary
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Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).

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Optimized Transfection Strategy Expression Electrophysiological Recording Recombinant Voltage-gated Ion Channels HEK-293T Cells In Vitro Cultured Human Embryonic Kidney Cells Glass Coverslips Density Electrophysiological Recording Contact Confounding Effects Plasma Membrane Whole-cell Patch Clamp Recording Detectable Currents Noise Levels Incubation Periods Membrane Expression Cell-coverslip Adhesion Membrane Stability Temperature Transfected Cells High Confluency Ion Channel Protein Expression Glass Coverslips Incubation Period Cell Attachment
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