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JoVE Journal
Biology

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Recapitulation of an Ion Channel IV Curve Using Frequency Components
 

Recapitulation of an Ion Channel IV Curve Using Frequency Components

Article DOI: 10.3791/2361-v 10:15 min February 8th, 2011
February 8th, 2011

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Summary

There are technical obstacles to measuring current flux through multiple ion channels simultaneously, and later discerning what portion of the transmembrane current is due to each channel type. To address this need, this method presents a way to generate the IV curve of individual channel types using specific frequency components.

Tags

Ion Channel IV Curve Impedance Spectroscopy Frequency Components Voltage Clamp Experiments Noise Function Model Cell Correlation Analysis Ion Channel Characterization Steady State Current Voltage Curve Multiple Channel Types
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