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Transfecting Human Neural Stem Cells with the Amaxa Nucleofector
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Transfecting Human Neural Stem Cells with the Amaxa Nucleofector

Article DOI: 10.3791/240-v • 11:25 min • July 19th, 2007
July 19th, 2007 •
1, 1
1Department of Pathology, University of California, Irvine (UCI)

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Introducing a gene of interest into a cell is a powerful method for elucidating its function in vivo. This protocol describes an efficient method of transfecting a culture of human neural stem/precursor cells (hNSPCs) using the Nucleofector electroporation apparatus made by Amaxa.

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Transfecting Human Neural Stem Cells Amaxa Nucleofector Transfection Cationic Lipid Transfection Reagents Cell Viability Transfection Efficiency Gene Gun Microinjection Viral Constructs Exogenous Gene Primary Cells Viral Vectors Safety Concerns Protocol Electrical Current Parameters Buffer Solutions Stable Transfection Electroporation
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