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Transfecting células-tronco neurais com o Nucleofector Amaxa
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Transfecting células-tronco neurais com o Nucleofector Amaxa

Article DOI: 10.3791/240-v • 11:25 min • July 19th, 2007
July 19th, 2007 •
1, 1
1Department of Pathology, University of California, Irvine (UCI)

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Introdução de um gene de interesse em uma célula é um método poderoso para elucidar a sua função in vivo. Este protocolo descreve um método eficiente de uma cultura de transfecting-tronco neurais humanas / precursor células (hNSPCs), utilizando o aparelho de eletroporação Nucleofector feita por Amaxa.

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Questão 6 Protocolos Basic Amaxa transfecção as células-tronco nucleofection
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