JoVE   
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Biology

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Neuroscience

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Immunology and Infection

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Clinical and Translational Medicine

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Bioengineering

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Applied Physics

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Chemistry

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Behavior

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You do not have subscription access to videos in this collection. Learn more about access.

Basic Methods in Cellular and Molecular Biology

You do not have subscription access to videos in this collection. Learn more about access.

Model Organisms I

You do not have subscription access to videos in this collection. Learn more about access.

Model Organisms II

You do not have subscription access to videos in this collection. Learn more about access.

Essentials of
Neuroscience

You do not have subscription access to videos in this collection. Learn more about access.

Essentials of Developmental Biology

You have subscription access to videos in this collection through your user account.

 JoVE Biology

Computer-Generated Animal Model Stimuli

1

1Center for the Integrative Study of Animal Behaviour, Macquarie University

Article
    Downloads Comments Metrics

    You must be subscribed to JoVE to access this content.

    This article is a part of   JoVE Biology. If you think this article would be useful for your research, please recommend JoVE to your institution's librarian.

    Recommend JoVE to Your Librarian

    Current Access Through Your IP Address

    You do not have access to any JoVE content through your current IP address.

    IP: 54.242.126.126, User IP: 54.242.126.126, User IP Hex: 921861758

    Current Access Through Your Registered Email Address

    You aren't signed into JoVE. If your institution subscribes to JoVE, please or create an account with your institutional email address to access this content.

     

    Summary

    Computer-generated stimuli using the Jacky dragon as a model.

    Date Published: 7/29/2007, Issue 6; doi: 10.3791/243

    Cite this Article

    Woo, K. L. Computer-Generated Animal Model Stimuli. J. Vis. Exp. (6), e243, doi:10.3791/243 (2007).

    Abstract

    Communication between animals is diverse and complex. Animals may communicate using auditory, seismic, chemosensory, electrical, or visual signals. In particular, understanding the constraints on visual signal design for communication has been of great interest. Traditional methods for investigating animal interactions have used basic observational techniques, staged encounters, or physical manipulation of morphology. Less intrusive methods have tried to simulate conspecifics using crude playback tools, such as mirrors, still images, or models. As technology has become more advanced, video playback has emerged as another tool in which to examine visual communication (Rosenthal, 2000). However, to move one step further, the application of computer-animation now allows researchers to specifically isolate critical components necessary to elicit social responses from conspecifics, and manipulate these features to control interactions. Here, I provide detail on how to create an animation using the Jacky dragon as a model, but this process may be adaptable for other species. In building the animation, I elected to use Lightwave 3D to alter object morphology, add texture, install bones, and provide comparable weight shading that prevents exaggerated movement. The animation is then matched to select motor patterns to replicate critical movement features. Finally, the sequence must rendered into an individual clip for presentation. Although there are other adaptable techniques, this particular method had been demonstrated to be effective in eliciting both conspicuous and social responses in staged interactions.

    Protocol

    Animation Design

    Object Scan

    Acquire taxidermic lizard. Scan the model with a Konica Minolta VI-9i. It produces a 3D object (*.lwo and *.obj files) in a single polygon mesh of 50,000 polygons. Lightwave® v8.3 has two programs used for 3D animation: Modeller and Layout. Lightwave® Modeller designs and manipulates the object. Lightwave® Layout program creates animation scenes. Lightwave® Modeller and Layout have three dimensions of positioning, orientation, and rotation: heading (Y), pitch (X), and bank (Z).

    Texture Acquisition

    Photograph a live lizard using an 12.8 megapixel Canon EOS 5D digital camera from two angles (frontal and orthogonal), three positions (frontal, orthogonal, ventral, and dorsal), and three body regions parts (head, body, tail, and limbs) over a white sheet of paper to be white balanced for standardized colour. Fuse photographs together in Adobe® Photoshop® Elements 3.0.

    Create an Atlas UV map in Lightwave® Modeller. The UV map breaks the object into strings of connecting polygons. Capture JPEG image of Atlas UV map with Grab V1.2 to create a separate JPEG image. Imbed as the background layer into Adobe® Photoshop® Elements 3.0.

    In the Lightwave® Modeller, highlight polygons on the Atlas UV map to identify specific areas on the lizard. Crop area and superimpose onto the background Atlas UV map JPEG. When all photographic fragments are layered onto the Atlas UV map JPEG, remove the background and create a single TIFF file. Import the TIFF file into Lightwave® Modeller and assign UV coordinates.

    Implanting Skelegons and Bones

    In Lightwave® Modeller, skelegons were designed and imbedded. Skelegons acted as placeholders for bones. Create 61 bones. Create skelegons to mimic the number of vertebrae in a Jacky dragon, with one large skelegon used to simulate the head. Create a virtual spinal column from the neck down to the end of the tail. Fuse the forelimbs, which consist of fours skelegons each, to spinal column. When all skelegons are created, send the entire object to Lightwave® Layout, where the skelegons are to be transformed into bones.

    Adding Weight Shading

    Independent weight maps have a value range from –100% to +100% to evenly distribute motion. Designated the limbs, tail, and head with positive (+) weight map values. Give the body a negative (-) weight map value.

    Stimulus Capture from Archival Video

    Archival lizard footage was shown using Sony MiniDV digital video recorder PAL player on a Sony Trinitron monitor to a live lizard held in its enclosure (for initial video acquisition, see Ord and Evans, 2002; Ord, Peters, Evans, & Taylor, 2002; Van Dyk and Evans, 2007). Responses of lizards were recorded using a Canon (MV650i) digital camcorder placed approximately on meter from the enclosure. Lizard size was scaled to the representative size of the perch on the display monitor. Export as individual sequential JPEG images in Apple Quicktime™ v7.0.

    Rotoscoping for Stimulus Replication

    ‘Rotoscoping’ is a technique where the object animation is superimposed frame-by-frame in the foreground of the background sequence (Gatsey, Middleton, Jenkins, & Shubin, 1999). Send the animation to Lightwave® Layout, where the light, camera, object, and background characteristics can be controlled for the scene. Select motor patterns from archival digital video and export as an image sequence (JPEG) using Apple QuickTime™ Player 7.0. The object must be in camera view. Import the first JPEG in the image sequence into the background. Manipulate the object using the bones and superimpose into the current position that reflects the background image. Each frame is to be ‘keyframed’, such that the position of the object is saved. Replace the background image with the next consecutive image and manipulate the object into the new position. Repeat the process until the sequence is finished.

    Rendering the Sequence for Completion

    Large sequence clips or batch processing are rendered using Render Farm Commander v2.9. Render Farm Commander (RFC) links up computers to produce a series of processors to aid in faster rendering. Four Apple Mac G5 dual-processors (eight threads) were used to distribute the rendering, thus reducing the time to four hours. All sequences were maintained at PAL-DV standard (720 ¥ 576 pixel resolution; 5:1 compression; horizontal resolution 575 lines; 25 frames s-1).

    Subscription Required. Please recommend JoVE to your librarian.

    Discussion

    Computer-generated animations are increasingly becoming a popular tool in which to investigate questions regarding communication in animal behaviour. By using animation techniques, researchers can focus on specific aspects of signal design, and manipulate features that previously were unable to be investigated due to limited technology. Although conventional staged animal encounters still demonstrate theoretical principles, animation techniques now provide a sophisticated tool for examining more detailed interactions.

    Subscription Required. Please recommend JoVE to your librarian.

    Disclosures

    Funding was supported by the CISAB and Department of Psychology Postgraduate Award of Macquarie University, and the Australian Research Council. Research was conducted in accordance to the ethical guidelines of the Macquarie University Animal Ethics Committee (2003/014) and the New South Wales National Parks and Wildlife Services (S11024).

    Acknowledgements

    I would like to thank Daniel Van Dyk from the Centre for the Integrative Study of Animal Behavour (CISAB) for much help in model design and access to archival video. Christopher Evans (CISAB) provided technical support, Daniel Warner (University of Sydney) provided the taxidermic model, and Richard Peters (Australian National University) provided early design features.

    Materials

    Name Type Company Catalog Number Comments
    New Item Digital camera Konica Minolta Holdings, Inc. VI-9i Used to acquire 3D mesh (done by New Dawn® - Bexley North, NSW Australia)
    Lightwave® 3D Software program NewTek Inc. v8.3 To create animation
    Canon Digital camera Canon, inc. EOS 5D 12.8 megapixels
    Grab Software program Apple v1.2 Extract texture from photographs and UV map
    Adobe® Photoshop® Elements Software program Adobe v3.0 Extract texture from photographs
    Render Farm Commander Software program Bruce Rayne© v2.9 Mass rendering across local area network
    Mac Pro Computer Apple Four dual processors (eight threads); Create and render animation
    Sony MiniDV Digital video recorder Sony Corporation GV-D300E Playback stimuli during stimulus acquisition
    Sony Trintron Colour viewfinder Sony Corporation PVM-14M2A One monitor for stimulus acquisition
    Canon Digital camcorder Canon, inc. MV650i Records behavior during stimulus acquisition

    References

    1. Gatesy, S. M., Middleton, K. M., Jenkins, F. A., Shubin, N. H. Three-dimensional preservation of foot movements in Triassic theropod dinosaurs. Nature. 399, 141-144 (1999).
    2. Newtek, Inc, Lightwave [8] Reference Manual. Newtek, Inc San Antonio, Texas (2004).
    3. Ord, T. J., Evans, C. S. Interactive video playback and opponent assessment in lizards. Behav. Process. 59, 55-65 (2002).
    4. Ord, T. J., Peters, R. A., Evans, C. S., Taylor, A. J. Digital video playback and visual communication in lizards. Anim. Behav. 63, 879-890 (2002).
    5. Rosenthal, G. G. Design considerations and techniques for constructing video stimuli. Acta Ethol. 3, 49-54 (2000).
    6. Van Dyk, D. A., Evans, C. S. Familiar-unfamiliar discrimination based on visual cues in the Jacky dragon, Amphibolurus muricatus. Anim. Behav. 74, 33-44 (2007).

    Comments

    0 Comments

    Post a Question / Comment / Request

    You must be signed in to post a comment. Please or create an account.

    Metrics

    Waiting
    simple hit counter