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Immunology and Infection

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JoVE Journal Immunology and Infection
Protocol for Recombinant RBD-based SARS Vaccines
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Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Article DOI: 10.3791/2444-v • 12:10 min • May 2nd, 2011
May 2nd, 2011 •
1, 1, 1, 1
1Lindsley F. Kimball Research Institute, New York Blood Center

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Summary
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This protocol describes a general procedure for studying recombinant receptor-binding domain (RBD)-based subunit vaccines against SARS. It includes methods for transfection and expression of RBD protein in 293T cells, immunization of mice with RBD and detection of neutralization activity of mouse sera using an established SARS pseudovirus neutralization assay.

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Recombinant RBD-based SARS Vaccines Protein Preparation Animal Vaccination Neutralization Detection Subunit Vaccines Mammalian Cell System Antigenicity Immunogenicity SARS-CoV RBD Protein Receptor Binding Neutralizing Antibodies Culture Supernatant Mammalian 293T Cells Transfection Method Calcium Phosphate Transfection
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