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Journal / Neuroscience / Fluorescence Recovery After Photobleaching (FRAP) …
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Neuroscience

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JoVE Journal Neuroscience
Fluorescence Recovery After Photobleaching (FRAP) van de fluorescentie gemerkte eiwitten in Dendritische stekels van Cultured hippocampus Neuronen
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Fluorescence Recovery After Photobleaching (FRAP) van de fluorescentie gemerkte eiwitten in Dendritische stekels van Cultured hippocampus Neuronen

Article DOI: 10.3791/2568-v • 06:36 min • April 16th, 2011
April 16th, 2011 •
1, 1, 1, 1
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

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FRAP is gebruikt om de mobiliteit van de groene fluorescentie Protein (GFP)-gemerkte eiwitten in gekweekte cellen te kwantificeren. We hebben de mobiele / immobiele fracties van de GFP onderzocht door het analyseren van de fluorescentie herstel na fotobleken percentage. In deze studie werd uitgevoerd op FRAP stekels van hippocampale neuronen.

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Neurowetenschappen Spine FRAP hippocampale neuronen live cell imaging eiwit mobiliteit
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