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JoVE Journal Bioengineering
Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)
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Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Article DOI: 10.3791/2605-v • 07:34 min • June 1st, 2011
June 1st, 2011 •
1,2, 1, 1, 1,3, 2, 2, 1, 3, 2, 1,3
1Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, 2Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, 3CT Surgery, Stanford University School of Medicine

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Summary
Automatic Translation

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

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Bioluminescence Imaging Assessment Immune Responses Implantation Engineered Heart Tissue Cardiac Tissue Engineering Scaffolding Strategies Native Scaffold Materials Bioartificial Scaffold Materials Cardiac Myocytes Hydrogels Fibrin Collagen Myocyte Monolayers Restoration Of Cardiac Function Myocardial Infarction Experimental Models Predictive Toxicology Substance Screening Disease Modelling Cell Survival Monitoring In-vivo Bioluminescence Imaging (BLI) Techniques Fibrin-based EHT Generation Transgenic Rat Strain Firefly Luciferase Expression ROSA/luciferase-LEW Tg Strain Omentum Implantation Immune Responses Assessment BLI Results Comparison With ELISPOT Technique
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