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Journal / Neuroscience / Transcriptoom Analyse van de individuele cellen
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Neuroscience

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JoVE Journal Neuroscience
Transcriptoom Analyse van de individuele cellen
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Transcriptoom Analyse van de individuele cellen

Article DOI: 10.3791/2634-v • 07:27 min • April 25th, 2011
April 25th, 2011 •
*1, *1, 1,2
1Department of Pharmacology, University of Pennsylvania, 2The Penn Genome Frontiers Institute, University of Pennsylvania
* These authors contributed equally

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In dit artikel beschrijven we een eenvoudige methode voor het verzamelen van enkele cellen van rat primaire neuronale culturen en de daaropvolgende transcriptoom analyse met behulp van ARNA versterking. Deze aanpak is generaliseerbaar naar een willekeurige cel type.

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Neurowetenschappen single-cel transcriptoom ARNA versterking RT-PCR moleculaire biologie genexpressie
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