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Journal / Neuroscience / L'analisi del trascrittoma di singole cellule
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Neuroscience

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JoVE Journal Neuroscience
L'analisi del trascrittoma di singole cellule
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L'analisi del trascrittoma di singole cellule

Article DOI: 10.3791/2634-v • 07:27 min • April 25th, 2011
April 25th, 2011 •
*1, *1, 1,2
1Department of Pharmacology, University of Pennsylvania, 2The Penn Genome Frontiers Institute, University of Pennsylvania
* These authors contributed equally

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In questo articolo viene descritto un metodo semplice per la raccolta di singole cellule di ratto colture primarie neuronali e analisi del trascrittoma con conseguente amplificazione ARNA. Questo approccio è generalizzabile a qualsiasi tipo di cellula.

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Neuroscienze Numero 50 cella singola trascrittoma ARNA amplificazione RT-PCR la biologia molecolare l'espressione genica
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