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Journal / Immunology and Infection / Kvantitativ bedömning av immunceller i den…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitativ bedömning av immunceller i den skadade ryggmärg med flödescytometri
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
Journal (Immunology and Infection)
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
Real-Time Polymorphonuclear Neutrophils (PMN) Chemotaxis Assay (Video) | JoVE
Journal (Immunology and Infection)
Real-Time Polymorphonuclear Neutrophils (PMN) Chemotaxis Assay (Video) | JoVE
 
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Kvantitativ bedömning av immunceller i den skadade ryggmärg med flödescytometri: en ny användning för en cell reningsmetod

Article DOI: 10.3791/2698-v • 08:01 min • April 9th, 2011
April 9th, 2011 •
1,2,3,4, 5, 1,2,3,4,6
1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California

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Kvantifiering av cellulär inflammation i skadade / patologiska CNS med flödescytometri kompliceras av lipid / myelin skräp som kan ha liknande storlek och granulering till celler, minskar känsligheten / noggrannhet. Vi har avancerat en metod cellberedningen att ta bort myelinet skräp och förbättra cell upptäckt med flödescytometri i den skadade ryggmärgen.

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Immunologi ryggmärgsskada cellulär inflammation neuroinflammation OptiPrep centrala nervsystemet neutrofiler makrofager mikroglia T-celler flödescytometri
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