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 JoVE Neuroscience

संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

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Epigenetics and Progenitor Cells Keystone, Fox Chase Cancer Center

 

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Cite this Article: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Zitserman, D., Roegiers, F. Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae. J. Vis. Exp. (51), e2706, doi:10.3791/2706 (2011).

Protocol: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Discussion: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Disclosures: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

ब्याज की कोई संघर्ष की घोषणा की.

Acknowledgements: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Materials: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

Name Company Catalog Number Comments
Scotch double stick tape
Glass slide
18mmX18mm coverslip
Forceps
Whatman paper
Silicone vacuum grease Dow Corning
5 ml syringe
Pipetter
Confocal or epifluorescence microscope

References: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

  1. Gho, M., Bellaiche, Y. & Schweisguth, F. Revisiting the Drosophila microchaete lineage: a novel intrinsically asymmetric cell division generates a glial cell. Development 126, 3573-3584 (1999).
  2. Roegiers, F., Younger-Shepherd, S., Jan, L. Y. & Jan, Y. N. Two types of asymmetric divisions in the Drosophila sensory organ precursor cell lineage. Nat Cell Biol 3, 58-67 (2001).
  3. Bellaiche, Y., Gho, M., Kaltschmidt, J. A., Brand, A. H. & Schweisguth, F. Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division. Nat Cell Biol 3, 50-57 (2001).
  4. Emery, G. & Knoblich, J. A. Endosome dynamics during development. Curr Opin Cell Biol 18, 407-415 (2006).
  5. Roegiers, F., Younger-Shepherd, S., Jan, L. Y. & Jan, Y. N. Bazooka is required for localization of determinants and controlling proliferation in the sensory organ precursor cell lineage in Drosophila. Proc Natl Acad Sci U S A 98, 14469-14474 (2001).
  6. Roegiers, F.et al. Frequent unanticipated alleles of lethal giant larvae in Drosophila second chromosome stocks. Genetics 182, 407-410, doi:genetics.109.101808 [pii] 10.1534/genetics.109.101808 (2009).
  7. Tong, X.et al. Numb independently antagonizes Sanpodo membrane targeting and Notch signaling in Drosophila sensory organ precursor cells. Mol Biol Cell 21, 802-810, doi:E09-09-0831 [pii] 10.1091/mbc.E09-09-0831 (2010).
  8. Jafar-Nejad, H.et al. Sec15, a Component of the Exocyst, Promotes Notch Signaling during the Asymmetric Division of Drosophila Sensory Organ Precursors. Dev Cell (2005).
  9. Karbowniczek, M.et al. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. J Clin Invest 120, 93-102, doi:40221 [pii] 10.1172/JCI40221 (2010).
  10. Coumailleau, F., Furthauer, M., Knoblich, J. A. & Gonzalez-Gaitan, M. Directional Delta and Notch trafficking in Sara endosomes during asymmetric cell division. Nature 458, 1051-1055, doi:nature07854 [pii] 10.1038/nature07854 (2009).
  11. Emery, G.et al. Asymmetric rab11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Cell 122, 763-773 (2005).
  12. Justice, N., Roegiers, F., Jan, L. Y. & Jan, Y. N. Lethal giant larvae acts together with numb in notch inhibition and cell fate specification in the Drosophila adult sensory organ precursor lineage. Curr Biol 13, 778-783 (2003).

Ask the Author: संवेदी अंग अग्रदूत साबित कोशिकाओं के बरकरार में लाइव सेल इमेजिंग ड्रोसोफिला Pupae

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