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JoVE Journal Immunology and Infection
Imuno-fluorescência Ensaio de Leptospirose superfície exposta Proteínas
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Imuno-fluorescência Ensaio de Leptospirose superfície exposta Proteínas

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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Um método eficiente para avaliar a exposição da superfície de proteínas leptospirose é descrito. O método é projetado especificamente para evitar a ruptura da membrana externa das células frágeis leptospirose. Esta técnica exige o emprego de vários controles negativos para avaliar a integridade da membrana externa e especificidade da reação de anticorpos.

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Imunologia Edição 53 Biologia Molecular Leptospira células intactas membrana externa de superfície exposta proteínas superfície imuno-fluorescência
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