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Immunology and Infection

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JoVE Journal Immunology and Infection
Yüzey maruz Leptospiral Proteinler immuno-floresans Testi
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Yüzey maruz Leptospiral Proteinler immuno-floresans Testi

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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Leptospiral proteinlerin yüzey pozlama değerlendirmek için etkili bir yöntem açıklanmıştır. Bu yöntem özellikle kırılgan leptospiral hücrelerin dış membran kesintileri önlemek için tasarlanmıştır. Bu teknik, dış membran ve antikor reaksiyonu özgüllüğü bütünlüğünü değerlendirmek için birkaç negatif kontroller istihdam gerektirir.

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İmmünoloji Sayı 53 Moleküler Biyoloji Leptospira sağlam hücreler dış membran yüzey maruz kalan proteinler yüzey immuno-floresans
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